Data/hora: 15/05/2024 01:38:08

Biblioteca(s): 	Embrapa Café.	
Data corrente: 	06/12/2023	
Data da última atualização: 	06/12/2023	
Autoria: 	IVAMOTO-SUZUKI, S. T.; CELEDÓN, J. M.; YUEN, M. M. S.; KITZBERGER, C. S. G.; DOMINGUES, D. S.; BOHLMANN, J.; PEREIRA, L. F. P.	
Afiliação: 	SUZANA TIEMI IVAMOTO-SUZUKI, UNIVERSIDADE ESTADUAL DE LONDRINA; JOSÉ MIGUEL CELEDÓN, UNIVERSITY OF BRITISH COLUMBIA; MACAIRE M. S. YUEN, UNIVERSITY OF BRITISH COLUMBIA; CÍNTIA SORANE GOOD KITZBERGER, INSTITUTO DE DESENVOLVIMENTO RURAL DO PARANÁ; DOUGLAS SILVA DOMINGUES, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; JÖRG BOHLMANN, UNIVERSITY OF BRITISH COLUMBIA; LUIZ FILIPE PROTASIO PEREIRA, CNPCa.	
Título: 	Functional characterization of ent-copalyl diphosphate synthase and kaurene synthase genes from coffea arábica L.	
Ano de publicação: 	2023	
Fonte/Imprenta: 	Journal of Agricultural and Food Chemistry, v. 71, n. 42, p. 15863-15873, 2023.	
Idioma: 	Inglês	
Conteúdo: 	The biochemical profile of coffee beans translates directly into quality traits, nutraceutical and health promoting properties of the coffee beverage. Ent-kaurene is the ubiquitous precursor for gibberellin biosynthesis in plants, but it also serves as an intermediate in specialized (i.e., secondary) diterpenoid metabolism that leads to a diversity of more than 1,000 different metabolites. Nutraceutical effects on human health attributed to diterpenes include antioxidant, anticarcinogenic, and anti-inflammatory properties. Cafestol (CAF) and kahweol (KAH) are two diterpenes found exclusively in the Coffea genus. Our objective was to identify and functionally characterize genes involved in the central step of ent-kaurene production. We identified 17 putative terpene synthase genes in the transcriptome of Coffea arabica. Two ent-copalyl diphosphate synthase (CaCPS) and three kaurene synthase (CaKS) were selected and manually annotated. Transcript expression profiles of CaCPS1 and CaKS3 best matched the CAF and KAH metabolite profiles in different tissues. CaCPS1 and CaKS3 proteins were heterologously expressed and functionally characterized. CaCPS1 catalyzes the cyclization of geranylgeranyl diphosphate (GGPP) to ent-copalyl diphosphate (ent-CPP), which is converted to ent-kaurene by CaKS3. Knowledge about the central steps of diterpene formation in coffee provides a foundation for future characterization of the subsequent enzymes involved in CAF and KAH biosynthesis.	
Thesaurus Nal: 	Bioinformatics; Biosynthesis; Caffeine; Coffee beans; Enzymes; Gene expression.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Café (CNPCa)	
Exemplares: 	CNPCa - (1722-1) - UPC - AP - DD -  -  -  - DIS